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• Dilute final milt/spermic urine sample by 100 (using 5 microliters sample).

Motility should be checked within 5 minutes of collection.

Motility assessment

Under a microscope count the amount of sperm that are:

1. Spiraling- Making a full circle and are still in spiral shape

2. Undulating- Vibrating slightly or slowly moving

3. Not moving/ broken- Completely still or fragmented. When counting fragmented pieces only count those with heads.

Stop counting once you reach 100 sperm. Motility count should be annotated as ##, ##, ## (spiraling, undulating, not moving)

<aside> 💡 View the video below if you would like a visual guide to determine the category of sperm.

</aside>

https://youtu.be/mVUzxCW1uxY

Concentration

• Use the hemocytometer (5 μL of diluted sample on the slide) to count the sperm in each 1 square mm. Do this for 5 squares and take the average of all 5 counts.

The average of [# sperm per each square] = [# average] x10^4 cells per mL

• Multiply by the original dilution and ensure that number is in scientific notation.

Osmolality

For Osmolality use 10 microliters of raw sample